Dihydrofolate reductaseDYR_ECOLIEC 1.5.1.3DHFRP0ABQ4DYR_ECOLI81175282Escherichia coliMISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERRDisorderedG-H loop142150DADAQNSHSComplex1DDSA1DDSB1DDRA1DDRBFunction arises via a disorder to order transitionSubstrate/ligand bindingX-ray crystallographyPaper14717591Schnell JR, Dyson HJ, Wright PEEffect of cofactor binding and loop conformation on side chain methyl dynamics in dihydrofolate reductase2004Biochemistry432374-839012674Sawaya MR, Kraut JLoop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence1997Biochemistry363586-603DisorderedF-G loop116132DAEVEGDTHFPDYEPDDComplex1DDSA1DDSB1DDRA1DDRBFunction arises via a disorder to order transitionSubstrate/ligand bindingX-ray crystallographyPaper14717591Schnell JR, Dyson HJ, Wright PEEffect of cofactor binding and loop conformation on side chain methyl dynamics in dihydrofolate reductase2004Biochemistry432374-839012674Sawaya MR, Kraut JLoop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence1997Biochemistry363586-603DisorderedMet20 loop924AVDRVIGMENAMPWNLComplex1DDSA1DDSB1DDRA1DDRBFunction arises via a disorder to order transitionSubstrate/ligand bindingX-ray crystallographyPaper14717591Schnell JR, Dyson HJ, Wright PEEffect of cofactor binding and loop conformation on side chain methyl dynamics in dihydrofolate reductase2004Biochemistry432374-839012674Sawaya MR, Kraut JLoop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence1997Biochemistry363586-603DisorderedMet20 loop1622MENAMPWComplex1RG7AFunction arises via a disorder to order transitionMolecular assemblySubstrate/ligand bindingX-ray crystallographyCaCl250imidazolepH 7.020imidazole (second aliquot)pH 7.260PEG 6000w/v25Paper9012674Sawaya MR, Kraut JLoop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence1997Biochemistry363586-60314717591Schnell JR, Dyson HJ, Wright PEEffect of cofactor binding and loop conformation on side chain methyl dynamics in dihydrofolate reductase2004Biochemistry432374-83Disordered9596GGComplex5DFRARelationship to function unknownUnknownUnknownX-ray crystallography277Paper9012674Sawaya MR, Kraut JLoop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence1997Biochemistry363586-60314717591Schnell JR, Dyson HJ, Wright PEEffect of cofactor binding and loop conformation on side chain methyl dynamics in dihydrofolate reductase2004Biochemistry432374-83DisorderedMet20 loop1620MENAMComplex5DFRA6DFRAFunction arises via a disorder to order transitionMolecular assemblySubstrate/ligand bindingX-ray crystallography277DHFR40 to 50 mg/mlX-ray crystallography298CaCl250imidazolepH 7.020imidazole (second aliquot)pH 7.260PEG 6000(w/v)25Paper9012674Sawaya MR, Kraut JLoop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence1997Biochemistry363586-60314717591Schnell JR, Dyson HJ, Wright PEEffect of cofactor binding and loop conformation on side chain methyl dynamics in dihydrofolate reductase2004Biochemistry432374-83PDB ID 5DFR had V10, L28, E120, D122, and D127 containing disordered side chains. PDB ID 6DFR had the side chains beyond the CB of E120 and D127 being disordered.Disordered6372SSQPGTDDRVComplex1DDRA1DDRB1DDSA1DDSBFunction arises via a disorder to order transitionUnknownUnknownX-ray crystallographyPaper9012674Sawaya MR, Kraut JLoop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence1997Biochemistry363586-60314717591Schnell JR, Dyson HJ, Wright PEEffect of cofactor binding and loop conformation on side chain methyl dynamics in dihydrofolate reductase2004Biochemistry432374-83