| General information | | DisProt: | DP00472 | | Name: | Peroxisomal biogenesis factor 5 | | Synonym(s): | Pex5p
| | First appeared in release: | Release 3.3 (06/23/2006) | | UniProt: | Q96FN7 | | UniGene: | Hs.567327 | | SwissProt: | | | TrEMBL: | Q96FN7 | | NCBI (GI): | 21361204 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 631 | | Percent disordered: | 51% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MAMRELVEAE CGGANPLMKL AGHFTQDKAL RQEGLRPGPW PPGAPASEAA SKPLGVASED - 60
ELVAEFLQDQ NAPLVSRAPQ TFKMDDLLAE MQQIEQSNFR QAPQRAPGVA DLALSENWAQ - 120
EFLAAGDAVD VTQDYNETDW SQEFISEVTD PLSVSPARWA EEYLEQSEEK LWLGEPEGTA - 180
TDRWYDEYHP EEDLQHTASD FVAKVDDPKL ANSEFLKFVR QIGEGQVSLE SGAGSGRAQA - 240
EQWAAEFIQQ QGTSDAWVDQ FTRPVNTSAL DMEFERAKSA IELQAELEEM AKRDAEAHPW - 300
LSDYDDLTSA TYDKGYQFEE ENPLRDHPQP FEEGLRRLQE GDLPNAVLLF EAAVQQDPKH - 360
MEAWQYLGTT QAENEQELLA ISALRRCLEL KPDNQTALMA LAVSFTNESL QRQACETLRD - 420
WLRYTPAYAH LVTPAEEGAG GAGLGPSKRI LGSLLSDSLF LEVKELFLAA VRLDPTSIDP - 480
DVQCGLGVLF NLSGEYDKAV DCFTAALSVR PNDYLLWNKL GATLANGNQS EEAVAAYRRA - 540
LELQPGYIRS RYNLGISCIN LGAHREAVEH FLEALNMQRK SRGPRGEGGA MSENIWSTLR - 600
LALSMLGQSD AYGAADARDL STLLTMFGLP Q
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| Functional narrative |
"Peroxisomal matrix proteins are synthesized in
cytosolic ribosomes and post-translationally
imported into the organelle. Two soluble receptor
proteins, Pex5p and Pex7p, promote the specific
targeting of these newly synthesized proteins into
the peroxisome. By far the majority of peroxisomal
matrix proteins use Pex5p as their cognate
receptor. These proteins possess the so-called
peroxisomal targeting signal (PTS) type 1, a
tripeptide located at their extreme C terminus
with the sequence S-K-L or a variant.... The C-terminal half of Pex5p, a region containing several TPR
motifs, interacts with the PTS1 sequence." (Carvalho et. al 2006)
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Pre-Molten Globule | | Name: | | | Location: | 1 - 324 | | Length: | 324 | | Region sequence: |
MAMRELVEAECGGANPLMKLAGHFTQDKALRQEGLRPGPWPPGAPASEAASKPLGVASED
ELVAEFLQDQNAPLVSRAPQTFKMDDLLAEMQQIEQSNFRQAPQRAPGVADLALSENWAQ
EFLAAGDAVDVTQDYNETDWSQEFISEVTDPLSVSPARWAEEYLEQSEEKLWLGEPEGTA
TDRWYDEYHPEEDLQHTASDFVAKVDDPKLANSEFLKFVRQIGEGQVSLESGAGSGRAQA
EQWAAEFIQQQGTSDAWVDQFTRPVNTSALDMEFERAKSAIELQAELEEMAKRDAEAHPW
LSDYDDLTSATYDKGYQFEEENPL | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises from the pre-molten globule state
| | Functional classes: | Chaperones
Molecular assembly
| | Functional subclasses: | Protein-protein binding
Self-transport through channel
| Detection methods:
- Far-UV circular dichroism (CD) (298 K; pH: 7.5; Sodium phosphate 10 mM)
- Gel filtration/size exclusion chromatography (298 K; pH: 7.5; DTT 1 mM; EDTA–NaOH 1 mM; NaCl 150 mM; Tris–HCl 50 mM)
- Fourier transform infrared spectroscopy (298 K; pH: 7.5; NaCl 150 mM; protein 1 mg/ml; Tris–HCl 50 mM)
- Sensitivity to proteolysis (273 K; 0.125 microg/ml - 1 microg/ml proteinase K; 5 micrograms protein)
- Thermal stability (95 °C for 30 min)
| References:
- Carvalho AF, Costa-Rodrigues J, Correia I, Costa Pessoa J, Faria TQ, Martins CL, Fransen M, Sa-Miranda C, Azevedo JE. "The N-terminal Half of the Peroxisomal Cycling Receptor Pex5p is a Natively Unfolded Domain." J Mol Biol. 2006; 356(4): 864-75.. PubMed: 16403517
- Dodt G, Gould SJ. "Multiple PEX genes are required for proper subcellular distribution and stability of Pex5p, the PTS1 receptor: evidence that PTS1 protein import is mediated by a cycling receptor." J Cell Biol. 1996; 135(6 Pt 2): 1763-74. PubMed: 8991089
- Otera H, Setoguchi K, Hamasaki M, Kumashiro T, Shimizu N, Fujiki Y. "Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import." Mol Cell Biol. 2002; 22(6): 1639-55. PubMed: 11865044
| Comments:
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| References |
- Purdue PE, Lazarow PB. "Peroxisome biogenesis." Annu Rev Cell Dev Biol. 2001; 17: 701-52. PubMed: 11687502
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