Peroxisomal targeting signal 1 receptor [Isoform 3]PEX5_HUMANPTS1 receptorPTS1RPeroxisome receptor 1Peroxisomal C-terminal targeting signal import receptorPTS1-BPPeroxin-5P50542-3Hs.567327PEX5_HUMAN119364633Homo sapiens (Human)MAMRELVEAECGGANPLMKLAGHFTQDKALRQEGLRPGPWPPGAPASEAASKPLGVASEDELVAEFLQDQNAPLVSRAPQTFKMDDLLAEMQQIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVTQDYNETDWSQEFISEVTDPLSVSPARWAEEYLEQSEEKLWLGEPEGTATDRWYDEYHPEEDLQHTASDFVAKVDDPKLANSEFLKFVRQIGEGQVSLESGAGSGRAQAEQWAAEFIQQQGTSDAWVDQFTRPVNTSALDMEFERAKSAIELQAELEEMAKRDAEAHPWLSDYDDLTSATYDKGYQFEEENPLRDHPQPFEEGLRRLQEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNQTALMALAVSFTNESLQRQACETLRDWLRYTPAYAHLVTPAEEGAGGAGLGPSKRILGSLLSDSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLSTLLTMFGLPQPeroxisomal matrix proteins are synthesized in cytosolic ribosomes and post-translationally imported into the organelle. Two soluble receptor proteins, Pex5p and Pex7p, promote the specific targeting of these newly synthesized proteins into the peroxisome. By far the majority of peroxisomal matrix proteins use Pex5p as their cognate receptor. These proteins possess the so-called peroxisomal targeting signal (PTS) type 1, a tripeptide located at their extreme C terminus with the sequence S-K-L or a variant. The C-terminal half of Pex5p, a region containing several TPR motifs, interacts with the PTS1 sequence. Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.Disordered - Pre-Molten Globule1324MAMRELVEAECGGANPLMKLAGHFTQDKALRQEGLRPGPWPPGAPASEAASKPLGVASEDELVAEFLQDQNAPLVSRAPQTFKMDDLLAEMQQIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVTQDYNETDWSQEFISEVTDPLSVSPARWAEEYLEQSEEKLWLGEPEGTATDRWYDEYHPEEDLQHTASDFVAKVDDPKLANSEFLKFVRQIGEGQVSLESGAGSGRAQAEQWAAEFIQQQGTSDAWVDQFTRPVNTSALDMEFERAKSAIELQAELEEMAKRDAEAHPWLSDYDDLTSATYDKGYQFEEENPLNativeFunction arises from the pre-molten globule stateMolecular assemblyChaperonesSelf-transport through channelProtein-protein bindingCircular dichroism (CD) spectroscopy, far-UV2987.5Sodium phosphate10Size exclusion/gel filtration chromatography2987.5DTT1EDTA-NaOH1NaCl150Tris-HCl50Fourier transform infrared spectroscopy (FTIR), aka infrared spectroscopy)2987.5NaCl150protein1Tris-HCl50Sensitivity to proteolysis2730.125 microg/ml - 1 microg/ml proteinase K5 micrograms proteinStability at thermal extremes36830 minPaper16403517Carvalho AF, Costa-Rodrigues J, Correia I, Costa Pessoa J, Faria TQ, Martins CL, Fransen M, Sa-Miranda C, Azevedo JEThe N-terminal half of the peroxisomal cycling receptor Pex5p is a natively unfolded domain2006J Mol Biol3564864-758991089Dodt G, Gould SJMultiple PEX genes are required for proper subcellular distribution and stability of Pex5p, the PTS1 receptor: evidence that PTS1 protein import is mediated by a cycling receptor1996J Cell Biol1356 Pt 21763-7411865044Otera H, Setoguchi K, Hamasaki M, Kumashiro T, Shimizu N, Fujiki YPeroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import2002Mol Cell Biol2261639-55